Project: “Interfaces nano” (nano-interfaces)
Project name: INTERFACES NANO
Subject: Reversible electroporation of cells by pulsed electric fields for implementation of nanoparticles
Start date: June 25, 2015
End date: June 25, 2016
Coordinator: IPREM - ECP
Scientific manager at the SIAME: Laurent PECASTAING
Permanent members of the SIAME involved: Marc RIVALETTO, Antoine SILVESTRE de FERRON
Type of contract: UPPA incentive-based call for projects
Partner(s): IPREM - LCABIE
Project description
Nanoshells are now considered as good potential candidates for photodynamic therapy for cancer owing to their optical resonance properties and a photothermal effect that kills the cancer cells by laser radiation. However, relatively large nanoparticles (of more than 50 nm) are required for good optical efficiency, but this affects the quality of cell absorption. To solve this problem, reversible electroporation – that consists of making the cells permeable (penetration of the agents) before allowing them to resume their initial permeability – can be considered as a promising tool to increase the absorption of nanoparticle cells. In this context, the “INTERFACES NANO” (nano-interfaces) project focuses on:
- The synthesis of hierarchical core-shell nanomaterials. The study concentrates on the shaping of the nanoparticles (as a film) in order to characterize the interfaces with the help of cross-sections, photoelectron spectroscopy (XPS, Ager) and electron microscopy analyses (SEM, TEM) of the materials.
- The design of pulsed electric field generators, so that the cells can be submitted to a configurable electric field (from around 1 to 10 kV/cm for periods of 1 to several tens of μs).
- Based on biological tests, the study of how the nanoparticles interact with the cancerous cells when pulsed electric fields are applied, allowing reversible electroporation of the cells owing to ICP-MS characterization of the nanomaterials in biological media.
